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. 2017 Oct 25;7:14048. doi: 10.1038/s41598-017-12968-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Myeloid progenitors are increased in C/EBPα conditional null mice. Bone marrow was isolated from CebpaΔ/Δ mice and littermate WT control mice. Single cell suspensions were made and cultured in MethoCult 3434 (A,B) or 3534 (C) semi-solid media. After 7–9 days incubation, colony types were evaluated and counted using an inverted microscope. The number of individual colony types and total colonies was quantified. Bone marrow was pooled from 3 animals each time and experiments were repeated twice. *p < 0.05. The data are presented as mean with SD.