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. 2017 Oct 25;7:14066. doi: 10.1038/s41598-017-14482-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect on SO EMG frequency and amplitude by manual acupuncture. (A) The frequency of SO EMG increased significantly after stimulation at ST25 and continued for 1 min (P < 0.05) compared to that before MA; compared to the pre-MA level, SO EMG frequency increased significantly after stimulation at LR14 (P < 0.01), but decreased significantly after stimulation at GB34 (P < 0.001) and at CO11 (P < 0.05). (B) Compared to the benchmark level before MA, the amplitude of SO EMG increased significantly after stimulation at ST25 (P < 0.01) and at LR14 (P < 0.01), but decreased significantly after stimulation at GB34 (P < 0.001) and at CO11 (P < 0.01). Duration for MA stimulation was 1 min each time. (C) Sample traces of SO EMG show that the frequency and amplitude increased significantly after MA stimulation at ST25 and at LR14, but decreased significantly after stimulation at GB34 and at CO11.