Figure 1.

Complementation of S. cerevisiae pho84 mutant with foxtail millet PHT1 transporters. Growth curves of S. cerevisiae pho84Δ mutant transformed with the indicated foxtail millet SiPHT1 transporters, empty vector (GFP-2 dotted line) and S. cerevisiae PHO84 (solid line) under 1.0 mM Pi (A) and 0.2 mM Pi Pi (B). Assay of growth of pho84Δ mutant cells transformed with SiPHT1;2, ScPHO84 or empty vector (GFP-2) in different concentrations of Pi (C). The OD₆₀₀ was measured after 20 hrs of growth. All the values are expressed as mean ± SD of 3 replicates and 2 repeats. The initial OD₆₀₀ cultures were adjusted to have the starting of 0.05.