Figure 7.

RT-PCR analysis and total and inorganic P content measurement in wild type plants and the 3 RNAi lines. (A) RT-PCR analysis of the 3 RNAi lines grown under low phosphorous (10 µM) using Si-actin, SiPHT1;2, SiPHT1;3 and SiPHT1;4 gene specific primers. WT = wild type plant (untransformed), SiPHT1;2-RNAi = transgenic plant downregulating SiPHT1;2, SiPHT1;3-RNAi = transgenic plant downregulating SiPHT1;3, SiPHT1;4-RNAi = transgenic plant downregulating SiPHT1;4. Lanes 2, 3 and 4 = amplification with SiPHT1;2, SiPHT1;3 and SiPHT1;4 gene specific primers respectively. Except SiPHT1;4-RNAi line, all the gels were cropped from various gels done at different time points. For SiPHT1;4-RNAi line, the individual lanes were from different gels which are indicated by delineating white lines. The individual gels were captured with different exposure times. T1 seedlings (4 weeks old) of RNAi lines grown under high Pi (B) and low Pi (C) were analysed for total and Pi contents. The total and Pi content of 2 weeks old (on hydroponics) seedlings were assayed using the same reported protocol⁸. The total height of the bars represents the total P. The lighter shading indicates that proportion of the total P that is Pi and the difference is the organic. Values were expressed as mean ± SD of 3 independent RNAi lines grown under low and high Pi. Values followed by the same letter are not significantly different (P > 0.001).