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Details of transgenic plants obtained and frequency of transformation for each RNAi line. For each treatment, three replicates were maintained. Values are expressed as the mean ± SD.
| Name of the transgenic line | Number of explants used | Total number of transgenic plants obtained (confirmed by PCR) | Frequency of transformation (%) |
|---|---|---|---|
| SiPHT1;2-RNAi | 126 | 35 | 9.2 ± 0.58 |
| SiPHT1;3-RNAi | 125 | 36 | 9.6 ± 0.37 |
| SiPHT1;4-RNAi | 121 | 33 | 9.0 ± 0.49 |
