Fig. 7.

Anti-HSV-2 activity of MZC and MZCL IVRs in macaques following repeated co-challenge with SHIV-RT and HSV-2. (a) HSV-2 infection and vaginal shedding were assessed by nested PCR in gD on vaginal fluid and cervical and vaginal tissue biopsies (n = 4 LNG, n = 12 MZCL, n = 4 placebo, n = 12 MZC). Evaluation commenced 4 wks after the last challenge. (b) Heat map depicting HSV-2 shedding in vaginal fluid observed over time for LNG, MZCL, placebo, and MZC groups from (a). Each row represents an animal. Shedding was measured at wks 4, 5, 6, 7, 8, and 9, or 10, and then again at 2, 6, 24, 30, 48, 54, and 72 h following biopsy. The legend shows the colors representing the fraction of replicate PCR reactions positive of 6 total with gray indicating not determined (nd), representing that no sample was available at that time point