Table 2.
Screening for NNRTI-resistance-associated mutations in macaques infected with SHIV-RT in the presence of IVRs
| IVR | Animal ID | Time point tested | L100, K101, K103, V108, E138, I178, V179, Y181, Y188, G190, P225 |
|---|---|---|---|
| MZCL | GK45 | Wk 8, Wk 12 | NAa |
| IC87 | Wk 6 | 0 (23)b | |
| HN43 | Wk 2 | 0 (24) | |
| GC05 | Wk 8 | NA | |
| MZC | EJ42 | Plasma: Ch 5–8, Wk 2, Wk 30 PBMC: Wk 30 |
Plasma: 67 (68) I178Vc
PBMC: 20 (23) I178V 1 (23) I178V + K101R Cultured PBMC: 19 (19) I178V |
| IT26 | Wk 6 | 0 (20) | |
| Stock SHIV-RT | 0 (32) |
aNA indicates that no RNA could be isolated from available plasma samples, likely due to low viral load and/or volume of plasma
bThe number of clones in which amino acid mutations conferring NNRTI resistance were detected. Parentheses indicate the total number of clones sequenced
cI178V was present in 23/23 clones of plasma virus from acute infection (pooled plasma from challenge 5–8) and 44/45 clones from chronic infection (23/23 at Wk 2 post-last challenge and 21/22 at Wk 30 post-last challenge)