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. 2017 Oct 17;21(3):745–757. doi: 10.1016/j.celrep.2017.09.074

Figure 2.

Figure 2

iRhom2 Phosphorylation Depends on MAP Kinases Downstream of Toll-like Receptor Signaling

(A) Schematics illustrating how signaling downstream of TLR3/4, GPCRs, and PMA converges on MAPK activation. Inhibitors are highlighted in red. Adapted from Goldsmith and Dhanasekaran, 2007, Steinberg, 2008, and Oda and Kitano (2006).

(B) A combination of ERK- and p38-MAPK inhibitors prevent endogenous iRhom2 phosphorylation in LPS-stimulated BMDMs.

(C) MAPK inhibitors (MEK1/2, JNK, and p38) prevent endogenous iRhom2 phosphorylation in poly(I:C)-stimulated BMDM.

Throughout, cells were pre-treated for 30 min with inhibitors (2.5 μM PD184352 [MEK1/2 inhibitor], 5 μM SP600125 [JNK inhibitor], 1 μM SB 202190 [p38 inhibitor]) followed by LPS (15 min) or poly(I:C) (60 min) treatment.