Figure 5.

USP7 Inhibitor Treatment Suppresses Wnt Activation in CID-Deleted APC Mutant CRCs

(A–C) Relative TOP/FOP activities of APC4 (A), SW480 (B), and Caco-2 (C) cells treated with HBX19818 at the indicated concentrations or DMSO as control.

(D) mRNA expression of the indicated Wnt target genes was analyzed by qRT-PCR in APC4 cells. Data are presented as fold change normalized to β-actin control in triplicate and are representative of at least three independent experiments.

(E–I) Relative TOP/FOP activities of APC3 (E), APC5 (F), APC2 (G), HCT116 (H), and DLD1 (I) cells treated with DMSO or HBX19818 at the indicated concentrations.

(J) MTT assay in the indicated cell lines treated with DMSO or HBX19818 at the indicated concentrations for 24 hr.

Error bars represent ± SE from at least three independent experiments (^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ns, not significant). See also Figure S5.