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. 2017 Oct 25;2(5):e00393-17. doi: 10.1128/mSphere.00393-17

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Copyright © 2017 Omrane et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Polymorphism discovery in 09-CB1 × IPO323 versus 09-ASA-3apz × IPO94269 bulks. (A) D values (GQ_R − GQ_S) plotted against their chromosomes (where IPO323 represents the sensitive parent) or contigs (where IPO94269 represents the sensitive parent) derived from 09-CB1 × IPO323 (upper panel) and 09-ASA-3apz × IPO94269 (lower panel). (B) Incrementing the bin size resolution of the regions concerned by the highest distortion, which shows a net increase of the D values (black line) between resistant bulk’s (red lines) and sensitive bulk’s GQ scores. Contigs showing the highest distortion (containing at least one polymorphic site with a D value of ≥0.5) from the 09-ASA-3apz × IPO94269 bulk sequencing were selected and aligned against the IPO323 genome. (C) Contig 1135 matching the region extending from 8 kb to 47 kb of chromosome 7 with the highest D values harbors 14 genes. SNPs and indels inducing nonsynonymous substitutions in the coding regions are indicated by stars.