MFS1 expression in Z. tritici field isolates with different promoter genotypes. MFS1 expression in selected field strains harboring type 1 to 3 inserts (blue, type I; red, type II; green, type III; violet, no insert). MFS1 expression was measured by qRT-PCR relative to three reference genes (β-tubulin, eF1a, and actin). Median values (n = 4 to 10) and interquartile range are indicated in the plot. ***, significant difference of the MFS1 expression levels between strains or groups of strains according to Kruskall and Wallis’ nonparametric statistical test with a risk threshold at 0.1%; *, significant differences at a risk threshold of 5%.