Fig. 5.

Wild-type rBRSV inhibits degradation of IκBα. Bovine primary CD14⁺ cells were mock-infected or infected with wt rBRSV or rBRSVΔSH (both m.o.i. of 3) for 3 h or treated with rboTNF-α for 10 min. Cell lysates were separated by SDS-PAGE and levels of IκBα determined by immunoblot. (a) Representative Western blot showing IkBα and endogenous α-tubulin. (b) Volumes of IκBα normalized to endogenous α-tubulin in each corresponding lane. Error bars indicate standard deviation (sd); * indicates P<0.05.