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HCV infection and replication in primary tupaia hepatocytes (PTHs). HCVcc was able to infect PTHs, but showed a lower level of replication compared with that in Huh7.5.1 cells (a). HCV RNA (b) and a specific protein (c) were positively detected in the culture supernatants and cell lysates of infected PTHs using RT–nPCR and Western blot, respectively.
