Fig. 3.

Bioanalyser-based analysis of RNA isolated from virions and dense bodies (DBs). To examine the small RNA species in virions (a) and DBs (b), a small RNA analysis kit (Agilent) was used. Representative electropherograms of RNA from virions and DBs show both miRNAs and small RNAs. (c) Mean percentages of miRNAs in virions and DBs; values are mean±sem of three experiments performed in triplicate. To inspect the total RNA species in virions (d) and DBs (e), an Agilent RNA 6000 Pico kit was used. Representative electropherograms of RNA from virions (d) and DBs (e) show very little 18S and almost no 28S rRNA, in contrast to the uninfected (f) and infected (g) MRC-5 cells. FU, fluorescence units; nt, number of nucleotides. Representative data from three experiments performed in triplicate are shown.