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. 2017 Jun 5;98(5):1058–1072. doi: 10.1099/jgv.0.000736

Fig. 5.

Fig. 5.

HCMV encoded miRNAs in virions, dense bodies (DBs), and infected and uninfected MRC-5 cells. To detect the miRNAs present in the virions and DBs, miRNA-specific cDNA was synthesised in the presence or absence of reverse transcriptase enzyme (RT), and TaqMan PCR was performed. TaqMan PCR analysis of 15 HCMV-encoded miRNAs, organised from the most abundant (top left) to the least abundant (bottom right), in virions, DBs, and uninfected and infected MRC-5 cells. The data were analysed with the 2−ΔCt method, where ΔC t=miRNA of interest C t value – Cel-miR39-3p spiked-in control C t value. Values are mean±sem of three experiments performed in triplicate.