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. 2017 Jun 5;98(5):1058–1072. doi: 10.1099/jgv.0.000736

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© 2017 The Authors

This is an open access article under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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Fig. 6. — HCMV-encoded miRNAs from virions and DBs can be delivered to host cells. UV-irradiated virions, or DBs, or mock samples were added to MRC-5 cell monolayers; non-UV-treated virus stock was used as a positive control. (a) Immunocytochemistry staining for HCMV IE to validate the effectiveness of UV treatment of virions and DBs. UV-irradiated virions and DBs failed to infect the MRC-5 cells; non-UV-irradiated viral stocks resulted in infection of MRC-5 cells. Grey, HCMV IE staining; blue, haematoxylin staining of nuclei. (b) Two HCMV-encoded miRNAs, miR-US25-1-5p and miR-UL112-3p, were detected in cells treated with UV-irradiated virions and DBs. The data were analysed with the 2^−ΔCt method, where ΔC _t=miRNA of interest C _t value – RNU48 C _t value. Values are mean±sem of three experiments performed in triplicate.