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. 2017 May 11;98(5):935–945. doi: 10.1099/jgv.0.000753

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© 2017 The Authors

This is an open access article under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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Fig. 2. — Transmission electron microscopy of WBV negatively stained particles (a, b, c) and infected Vero cells (d, e, f). (a) Virion of icosahedral-spherical shape, with an apparently amorphous envelope; (b) heterogeneity in particle size; (c) virus particle in which regularly spaced, envelope projections (right side) contrast with the fuzzy appearance of the envelope (left side); (d) transverse sections through characteristic tubular elements (arrows) forming within Golgi cisternal stacks, which are in close proximity to mitochondrial profiles and distended rough endoplasmic reticulum (RER); (e) developing virions (arrows) within a Golgi-derived vesicle, approaching the plasmalemma (PL) for exocytosis. Note the organelle arrangement with the nucleus (N) and numerous mitochondrial profiles in juxtaposition to the disrupted Golgi body; (f) mature virions (arrows) with glycoprotein envelope spikes, still within an endomembrane that appears continuous with those of the tangentially sectioned mitochondrial profiles. Scale bars: (a, b, c) 12 nm; (d, f) 100 nm; (e) 250 nm.