Table 1. Correlation of real-time RT-PCR C _t values to TCID₅₀ .

Real-time RT-PCR C t value	Corresponding TCID50 equivalent value
<16	>1×106.75
16–19	1×105.75 to 1×106.75
19–23	1×104.75 to 1×105.75
23–27	1×103.75 to 1×104.75
27–30	1×102.75 to 1×103.75
30–34	1×101.75 to 1×102.75
34–38	1×100.75 to 1×101.75
38–40	1×100.25 to 1×100.75
40–42	1×10−0.25 to 1×100.25
42–45	1×10−0.75 to 1×10−0.25