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a) Influence of the substrate concentration on the changes in fluorescence of the microgel. b) Average transient periods of intensity as a function of the urea concentration. c) Reversible fluorescence changes in time following repeated additions of chemical fuels (60 mm urea). d) Average transient periods of intensity within five cycles (60 mm urea). Experimental conditions: 0.6 mg mL−1 urease, 9 mm CA/Na3C (9:1) buffer.
