Table 2.
Primer name | Direction | Sequence (5′ to 3′)a |
---|---|---|
SK‐Sep‐14 | Sense | CATTTGCGGCTGTCTCGAAATCGACGGAAGGCAGTCGACGCCAGATGATGG |
SK‐Sep‐16 | Sense | ATGGTGAGCAAGGGCGAGGAG |
SK‐Sep‐47 | Antisense | GGCGATGGTGGTATGCGGATG |
SK‐Sep‐78 | Antisense | CCACAAGATCCTGTCCTCGTCCGTCGTCGCTTACTTGTACAGCTCGTCCATGC |
SK‐Sep‐156 | Sense | CATCACTCACATCCGCATACCACCATCGCCATGGGATATTACGACGAAGACGG |
SK‐Sep‐157 | Antisense | GGTGAACAGCTCCTCGCCCTTGCTCACCATCAAGCGGCTACCGTGGACGAC |
Italics indicates part of the primer that is complementary with another DNA fragment to be ligated by homologous recombination in Saccharomyces cerevisiae.