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Complementation of Zn and Fe yeast uptake mutants with TaZIP genes.
The yeast Zn uptake mutant zrt1/zrt2 (a–d) and the Fe uptake mutant fet3/fet4 (e–g) were transformed with five wheat TaZIP genes, shown here with empty vector controls (e.v.) in both the mutant and wild‐type yeast strains. Each spot is a dilution of the culture starting on the left of each plate (undiluted, 1:2, 1:10, 1:100, 1:1000) with the contents of selective media described above each plate.
