Figure 1. Ntn4 expression is essential for osteoblast differentiation. (A) The expression of Bglap and Ntn4 mRNA in MC3T3-E1 during osteoblast differentiation in the presence of ascorbic acid and β-glycerophosphate at 7 and 14, and 21 days estimated by qPCR analysis. (B) Effect of RNA interference to Ntn4 (MSS226597) in MC3T3-E1 at 3 days estimated by qPCR analysis: (left panel) Ntn4, (right panel) ALP. Data are calculated from three repeated experiments. si-control (si-CTL), Stealth RNAi Negative Control Duplex; si-Ntn4, Stealth RNAi to Ntn4. #p<0.05; ##p<0.01. Data are expressed as the means±S.E.M.