Endochondral ossification. (A) Localization
of CHP binding in a
sagittal section of an 18 d.p.c. mouse embryo (E18) double stained
with B-CHP (detected by AlexaFluor647-streptavidin, orange) and an
anti-collagen I antibody (detected by AlexaFluor555-labeled donkey
anti-rabbit IgG H&L, cyan). mx, maxilla; md, mandibular bone; bp, basisphenoid bone; bo, basioccipital bone; vc, vertebral column; rb, rib; h, hipbone; d, digital bones. (B) Magnified views of the basioccipital bone (bo) beside the C1 vertebra (v) (top images)
and the manubrium sterni (ms, bottom images) in the
sagittal section of a 17 d.p.c. mouse embryo (E17) stained in the
same fashion. High levels of CHP binding are found in the hypertrophic
zone surrounding the newly deposited collagen I bone matrix (yellow
arrow heads), which is visualized by the anti-collagen I antibody.
Scale bars: 3 mm (A), 0.5 mm (B).