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. 2017 Sep 6;11(10):9825–9835. doi: 10.1021/acsnano.7b03150

Figure 6.

Figure 6

Endochondral ossification. (A) Localization of CHP binding in a sagittal section of an 18 d.p.c. mouse embryo (E18) double stained with B-CHP (detected by AlexaFluor647-streptavidin, orange) and an anti-collagen I antibody (detected by AlexaFluor555-labeled donkey anti-rabbit IgG H&L, cyan). mx, maxilla; md, mandibular bone; bp, basisphenoid bone; bo, basioccipital bone; vc, vertebral column; rb, rib; h, hipbone; d, digital bones. (B) Magnified views of the basioccipital bone (bo) beside the C1 vertebra (v) (top images) and the manubrium sterni (ms, bottom images) in the sagittal section of a 17 d.p.c. mouse embryo (E17) stained in the same fashion. High levels of CHP binding are found in the hypertrophic zone surrounding the newly deposited collagen I bone matrix (yellow arrow heads), which is visualized by the anti-collagen I antibody. Scale bars: 3 mm (A), 0.5 mm (B).