Figure 2.

Intracellular distribution of endogenous and overexpressed V5-tagged TCF4 proteins in rat primary neurons. a, Neurons were cotransfected with Tcf4 exon 12 or 20 specific siRNAs and an EGFP construct for identification of transfected cells (marked with asterisks); mock and scrambled siRNA transfections were performed in control. Arrows indicate nonspecific staining of glial processes with TCF4 antibodies. b, Neurons were transfected with TCF4–V5 constructs. Localization pattern is indicated at the right. n, Nuclear; n + c, nuclear and cytoplasmic. In a and b, tubulin III and DNA were stained to label neurons and nuclei (encircled in a), respectively.