Figure 7.

The effect of SCZ-linked TCF4 missense variations on the transcriptional activity of TCF4 in neurons. Reporter assay with primary neurons transfected with WT or mutant EF1α-TCF4-B⁻ vectors, firefly luciferase construct carrying 12 μE5 E-boxes in front of TK promoter, and Renilla luciferase construct with PGK1 promoter. Transfected neurons were left untreated or treated with 25 mm KCl for 8 h. Data are presented as fold induced levels above the signals obtained from untreated cells overexpressing WT TCF4-B⁻. Shown are the mean results from 5 independent experiments (except for P156T^+KCl, in which n = 4). Error bars indicate SEM. *p < 0.05; **p < 0.01; two-way ANOVA followed by Dunnett's post hoc tests for comparisons with untreated or KCl-treated WT. RLU, Relative luciferase units.