Table 2.

Biological pathways enriched in genes showing differential methylation in maternal separation versus control WKY rat hippocampal tissue.

Biological Process Pathways		p-value	enrichment ratio	Genes in Pathway
1	positive regulation of cell proliferation	0.008	5.42	Sphk2, Nf1, Nacc1, Cdh13, Peli1, Efnb1, Insr, St8sia1, Ntn1
2	cell proliferation	0.015	3.42	Sphk2, Nf1, Nacc1, Cdh13, Peli1, Efnb1, Insr, St8sia1, Ntn1, Ephb1, Vpreb1, Fgfrl1
3	transmembrane receptor protein tyrosine kinase signaling pathway	0.023	6.62	Cdh13, Efnb1, Insr, Ephb1, Fgfrl1, Atxn1
4	regulation of cell proliferation	0.023	3.53	Sphk2, Nf1, Nacc1, Cdh13, Peli1, Efnb1, Insr, St8sia1, Ntn1, Fgfrl1
5	protein modification process	0.023	3.53	Eya2, Ephb1, Nf1, Peli1, Hus1, Insr, Lrp8, St8sia1, Fgfrl1, Prdm5, Hs3st5, Hecw2
6	macromolecule modification	0.090	2.28	Eya2, Ephb1, Nf1, Peli1, Hus1, Insr, Lrp8, St8sia1, Fgfrl1, Prdm5, Hs3st5, Hecw2
7	locomotion	0.090	2.20	Ephb1, Nf1, Cdh13, Efnb1, Insr, Lrp8, Hs3st5, Ntn1
8	cellular protein modification process	0.090	3.19	Eya2, Ephb1, Nf1, Peli1, Hus1, Insr, Lrp8, St8sia1, Fgfrl1, Prdm5, Hs3st5, Hecw2
9	synaptic transmission	0.090	2.28	Grik4, Ephb1, Nf1, Atxn1, Lrp8
10	regulation of locomotion	0.090	3.98	Nf1, Hs3st5, Cdh13, Insr, Ntn1

This list represents the top ten gene onology (GO) biological processes (p < 0.1) along with their associated p-value for enrichment, ratio of enrichment (calculated based on number of genes observed versus those expected), and the genes that appeared within each pathway. The gene list used for this analysis included those containing differentially methylated regions (DMRs) that occurred within the gene body or within 2 kb upstream of gene (p < 0.1).