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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Endod. 2017 Aug 1;43(9 Suppl):S25–S30. doi: 10.1016/j.joen.2017.06.006

Figure 1.

Figure 1

Effect of LRP6 and Frizzled6 on VEGF or Wnt1 signaling in DPSC cells. (A) Western blots to evaluate the effectiveness of LRP6 or Frizzled6 silencing in DPSC cells. We tested several shRNA sequences for Frizzled6 (#30,31,33,52) and for LRP6 (#1–5). (B) Fluorescence image to evaluate the effectiveness of lentivirus-mediated transduction of shRNA-LRP6 or shRNA-Frizzled6 into DPSC cells. All lentiviral vectors used here (including controls) contained EGFP. Photomicrographs were taken at 200× magnification. (C) Western blots depicting effect of increasing concentrations of rhVEGF165 or rhWnt1 on phosphorylated and total β-catenin, GSK-3β, and AKT.