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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Endod. 2017 Aug 1;43(9 Suppl):S25–S30. doi: 10.1016/j.joen.2017.06.006

Figure 4.

Figure 4

LRP6 silencing inhibits endothelial differentiation of DPSC in vivo. (A) Tooth slices/scaffolds seeded with LRP6-silenced DPSC, Frizzled6-silenced DPSC, or vector control DPSC were transplanted into the subcutaneous space of immunodeficient mice. After 28 days, tooth slice/scaffolds were retrieved, fixed, and analyzed by hematoxilin-eosin staining (200×), or immunohistochemistry with EGFP antibody (400×). (B) Graph depicting the density of EGFP-positive blood vessels (i.e. generated by DPSC cells) in 6 tooth slice/scaffolds per experimental condition. Blood vessels were counted in 10 microscopic fields/specimen at 200× magnification. Different letters represent p<0.05.