Table 4.

Performance of MegaGTA, Xander and MEGAHIT on the rhizosphere soil metagenomic sample

	MegaGTA		Xander		MEGAHIT
Gene rplB
Cluster Identity	99%	95%	99%	95%	99%	95%
# of gene contigs aligned by Framebot	17,668	5079	15,933	4237	578	465
Total length (bp)	13.9 M	3.96 M	12.5 M	3.32 M	378 k	311 k
Median length (bp)	822	822	822	822	639	660
# of matched reference genes	491	427	456	385	208	193
Median % aa identity	76.73	76.00	77.46	76.90	77.50	77.07
Gene nifH
Cluster Identity	99%	95%	99%	95%	99%	95%
# of gene contigs aligned by Framebot	33	11	31	10	9	5
Total length (bp)	27.8 k	9225	25.3 k	8412	7368	4464
Median length (bp)	888	888	882	883.5	930	930
# of matched reference genes	13	10	12	8	8	5
Median % aa identity	91.55	90.54	92.96	91.19	85.14	83.99
Gene nirK
Cluster Identity	99%	95%	99%	95%	99%	95%
# of gene contigs aligned by Framebot	1336	392	1242	336	203	179
Total length (bp)	1.09 M	321 k	1.02 M	277 k	170 k	153 k
Median length (bp)	687	787.5	690	748.5	735	750
# of matched reference genes	55	53	50	47	71	66
Median % aa identity	89.29	86.61	89.06	87.30	66.41	65.97