Table 1.
Infection efficiency of various Phytophthora species and strains on MsK8 cells
| Percentage | P. infestans | P. capsici | P. palmivora | P. sojae | P. parasitica | ||||
|---|---|---|---|---|---|---|---|---|---|
| 14-3-GFP | IPO-C | T20-2 | LT263 | LT3239 | LT51 | GFP3 | P6497 | H1111 | |
| Infected MsK8 cellsa | 36.4 ± 1.2 | 25.4 ± 0.4 | 16.6 ± 2.3 | 7.0e ± 4.5 | 0 | 34.0 ± 3.4 | 39.4 ± 1.3 | 0 | 0 |
| Primary infectionb | 73.6 ± 3.6 | 73.2 ± 4.0 | 52.0 ± 2.5 | – | – | 55.3 ± 2.8 | 26.4 ± 2.1 | – | – |
| Secondary infectionc | 26.4 ± 1.4 | 26.8 ± 2.7 | 48.0 ± 2.6 | – | – | 44.7 ± 1.1 | 73.6 ± 2.6 | – | – |
| Cells containing haustoriad | 21.4 ± 1.1 | 11.8 ± 1.8 | 7.1 ± 0.9 | – | – | 7.0 ± 0.6 | 7.1 ± 1.5 | – | – |
The efficiency was quantified by determining the percentage of infected cells at 16 h post inoculation. For each sample a total of 500 cells was monitored in triplicate
aCells that had been penetrated by Phytophthora were counted as infected
bInfected cells due to primary infection i.e. penetrated by germ tubes emerging from cysts
cInfected cells due to secondary infection i.e. penetrated by hyphae expanding from a neighbouring infected cell
dInfected cells containing haustoria
eAttachment of hyphae and scarce initial penetrations with no further growth of hyphae in the cell