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. 2017 Oct 25;48:66. doi: 10.1186/s13567-017-0474-x

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characterization of JOL1950 ghosts constructed by endolysin expression. A Western blot analysis. Endolysin expressed in JOL1950 was confirmed by rabbit anti-endolysin polyclonal antibodies. Black arrows indicate the expected size of endolysin (~17 kDa). Lane M, size marker; lane 1, JOL1950 grown in the presence of l-arabinose at 27 °C; lane 2, JOL1950 ghosts produced under lysis conditions. Characterization of JOL1950 S. Typhimurium ghost cells by scanning electron microscopy (SEM). B Intact JOL1950 cells before lysis. C JOL1950 ghost cells after 36 h of lysis. Arrowheads indicate lysis transmembrane tunnels.