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. 2017 Oct 25;48:66. doi: 10.1186/s13567-017-0474-x

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Assessment of serum IgG antibody responses post-immunization. Titers of serum IgG, IgG1, and IgG2a against a Salmonella-specific antigen OMP in immunized mice were measured by ELISA assay. Mice were subcutaneously injected with lysed JOL1950 while non-stimulated mice were inoculated with 100 μL of sterile PBS. Data are shown as mean ± standard error (SE). NS; non-stimulated, *P < 0.05, (vs. NS).