Table 1.
Bacterial strains and plasmids utilized in this study
| Strain/plasmids | Description | References |
|---|---|---|
| E. coli | ||
| JOL232 (χ6212) | F-λ-φ80 Δ(lacZYA-argF) endA1 recA1 hadR17 deoR thi-1 glnV44 gyrA96 relA1 ΔasdA4 | Lab stock |
| Salmonella Typhimurium | ||
| JOL 401 | Wild type isolate from porcine, challenge strain | Lab stock |
| JOL1311 | Δasd, used as base vaccine strain | Lab stock |
| JOL1950 | JOL1311 containing pJHL464 | This study |
| Plasmids | ||
| pJHL172 | asd + vector, pBR ori plasmid harboring cI857/λPR promoter, araC ParaBAD, phiX174 lysis gene E | [22] |
| pJHL319 | T-easy vector harboring E gene ghost cassette with the convergent promoter system | This study |
| pJHL360 | T-easy vector harboring the R ghost cassette with the convergent promoter system | This study |
| pJHL464 | asd+ vector, pBR ori plasmid harboring cI857/λPR promoter, araC ParaBAD, the R ghost cassette composed of S, R and R1/Rz genes | This study |