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. 2017 Oct 25;48:67. doi: 10.1186/s13567-017-0471-0

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The frequency of residues at positions 283 and 526 in the PB2 of various subtypes of IAVs isolated from different hosts. Full-length PB2 sequences of IAVs isolated from avian, swine and human were obtained from the Influenza Sequences Database. Sequence alignment was performed by the Clustal W alignment method using the Megalign program. The percentage of the isolates possessing the indicated residues within each subtype was calculated and indicated by the colored area.