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Extent of PC:Cholesterol (2:1 mole ratio) liposomes association with five cancer cell lines (MCF-7, MDA- MB-231, PC3, H460 and SK-Mel-5) and three immortalized cell lines (WMPY, 16-HBE and ARPE-19). Liposomes labeled with 0.01% DID were incubated with all cell lines for 4 hrs at 37°C. Extent of association was measured using flow cytometry. Association was quantified by monitoring the percentage of cells that had increased fluorescence when compared to control cells (“Positive”).
