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Effect of pH on exosome uptake. Top: Exosomes derived from MDA-MB-231 cells cultured at pH 7.4, 6.8 and 6.3 were incubated with MDA-MB-231 cells growing at pH 7.4, 6.8, and 6.3. Bottom: Exosomes derived from PC3 cells cultured at pH 7.4, 6.8 and 6.3 were incubated with PC3 cells growing at pH 7.4, 6.8, and 6.3. All exosomes were labeled with 0.01% DID. For each panel, association with pH 6.3 exosomes was significantly less than with pH 7.4 exosomes (p<.05).
