Table 2.
Extent of exosome association with recipient cells as seen in Figures 3 and 4 are compiled for comparison. Adherence/internalization experiments of liposomes and exosomes were performed simultaneously to prevent day-to-day variations in the extent of association. Bolded numbers indicate association of “daughter” exosomes by the “parent” cell line. Both liposome association and exosome association values for both cancer and immortalized cell lines are averaged. Data from Figure 4 is further normalized as described in the text to account for differences in non-specific association when comparing exosome association between cancer and immortalized cell lines.
| Cell Line | Percent Positive | Normalized Percent Positive | ||||||
|---|---|---|---|---|---|---|---|---|
| Liposome | MCF-7 Exo | MDA Exo | PC3 Exo | Relative Liposome Uptake | MCF-7 Exo | MDA Exo | PC3 Exo | |
| MCF-7 | 24.9 | 34.1 | 32.4 | 16.4 | 0.9 | 38.6 | 36.7 | 18.6 |
| MDA-MB-231 | 28.2 | 40.6 | 18.0 | 17.2 | 1.0 | 40.6 | 18.0 | 17.2 |
| PC3 | 22.1 | 32.3 | 24.3 | 18.0 | 0.8 | 41.9 | 31.0 | 23.0 |
| H460 | 38.9 | 45.1 | 32.2 | 20.3 | 1.4 | 32.7 | 23.3 | 14.7 |
| SK-Mel-5 | 44.9 | 39.8 | 22.1 | 16.9 | 1.6 | 25.0 | 13.9 | 10.6 |
| WPMY | 21.6 | 29.8 | 14.7 | 9.5 | 0.8 | 38.9 | 19.2 | 12.4 |
| 16-HBE | 31.7 | 15.5 | 7.7 | 6.1 | 1.1 | 13.8 | 6.9 | 5.4 |
| ARPE-19 | 21.3 | 45.7 | 16.2 | 14.0 | 0.8 | 60.5 | 21.4 | 18.5 |
| Average | 29.2 | 35.4 | 21.0 | 14.8 | – | 36.5 | 21.3 | 15.1 |
| Average Liposome Cancer % Positive | 31.8 | Avenge Normalized Cancer % Positive | 25.7 | |||||
| Average Liposome Immortalized & Positive | 24.9 | Average Normalized Immortalized % Positive | 21.9 | |||||
| Auerage Exosome Cancer % Positive | 27.3 | Average Normalized WPMY and ARPE-19 % Positive | 28.5 | |||||
| Average Exosome Immortalized % Positive | 17.7 | |||||||
| Average Exosome WPMY and ARPE-19% Positive | 21.7 | |||||||
| Average Liposome WPMY and ARPE-19% Positive | 21.5 | |||||||