Figure 2. “Puffed” neutrophils are easily destroyed by physical stimuli.

Freshly isolated human neutrophils were cultured as described in Figure 1. (A) After staining with Annexin V-FITC and PI, cells were gently pipetted up and down using P1000 tip 10 times. Bars indicate 20 μm. (B) After culturing, cells were fixed with 4% Paraformaldehyde (PFA) for 30 min at room temperature, then gently pipetted up and down using P1000 tip 10 times. Arrows indicate “puffed” cells. Bars indicate 20 μm.