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. Author manuscript; available in PMC: 2017 Oct 26.
Published in final edited form as: Kidney Int. 2016 Dec 15;91(4):856–867. doi: 10.1016/j.kint.2016.10.007

Figure 1. Schematic description of it-ACE mice.

Figure 1

Two types of transgenic constructs were used to make these mice. Construct 1 is the kidney specific (Ksp)-cadherin/β-globin promoter driving the transcription factor tetracycline transactivator (tTAoff) only in renal tubular epithelial cells. Construct 2 possesses a modified cytomegalovirus promoter that, in the presence of tTAoff, produces the tdTomato fluorescent protein and a short hairpin RNA (shRNA) designed to silence angiotensin converting enzyme (ACE) mRNA. Renal confocal microscopy shows a glomerulus (negative) surrounded by tdTomato-positive tubules (orange). Green color is normal tubular autofluorescence. Oral administration of doxycycline (200 μg/ml in the drinking water) inactivates the tTAoff transcription factor, prevents shRNA production, and restores ACE expression. IRES, internal ribosome entry site.