Fig. 1. HPLC chromatograph of 2 mM m6A incubated with 200 mM H2O2 and 1 M NH4HCO3 at 37 °C for 0 h (a), 1 h (b) and 24 h (e). As shown in the HPLC profiles, when the reaction proceeded for 1 h, one major product (A) was produced, accompanied by three intermediates (hm6A, f6A, oxm6A). The synthesized hm6A (c) and f6A (d) standards have the same retention time as two of the new peaks in the reaction mixture. Because hm6A and f6A are unstable, they can coexist with A during HPLC analysis.
