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Phosphorylated and total levels of AMPK and RAPTOR were detected by western blot in protein samples prepared from C57Bl6/J mouse livers snap frozen in liquid nitrogen at the indicated times after intraperitoneal injection with metformin at the indicated ZTs. pAMPK and pRAPTOR represent phosphorylation on T172 and S792 respectively. Each lane represents a unique animal. A representative experiment is shown.
