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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: J Biol Rhythms. 2017 Jun 15;32(4):345–358. doi: 10.1177/0748730417710348

Figure 5. The liver clock modulates transporter expression.

Figure 5

mRNA expression of Bmal1 (A), Slc47a1 (B), Slc22a1 (C), Slc16a1 (E) and Slc16a7 (F) detected by qPCR in mouse livers collected at the indicated ZTs. N=6–9 samples per condition. *** P < 0.001, **** P < 0.0001 vs. control; ## P < 0.01, #### P < 0.0001 vs. ZT7 by post-hoc testing corrected for multiple comparisons. (D) Metformin detected by mass spectrometry in control and L-Bmal1−/− mouse livers snap frozen in liquid nitrogen 20 minutes after intraperitoneal injection with metformin. (G) Results of two-way ANOVA analyses of gene expression data presented in (A–C, E–F). * P < 0.05, ** P < 0.01, ** P < 0.001, **** P < 0.0001 for an effect of genotype, Zeitgeber Time, or interaction between the two as indicated.