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. Author manuscript; available in PMC: 2018 Oct 23.
Published in final edited form as: Curr Protoc Neurosci. 2017 Oct 23;81:2.28.1–2.28.12. doi: 10.1002/cpns.35

Figure 2. TrueBlack reduces red blood cell fluorescence in immunostained tissue.

Figure 2

E12.5 fixed tissue sections were immunostained for GnRH and treated with 1X TrueBlack for 3 minutes or 0.1% Sudan Black for 30 minutes. Nasal tracks with stained GnRH neurons (green, open arrows) were imaged at green (488 nm), red (555 nm), and blue (405 nm) wavelengths. Untreated tissue contained many red blood cells (solid arrows) that fluoresced across all three wavelengths. Treatment with TrueBlack and Sudan Black B reduced autofluorescence, but Sudan Black B enhanced background fluorescence (asterisk).