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. Author manuscript; available in PMC: 2018 Oct 23.
Published in final edited form as: Curr Protoc Neurosci. 2017 Oct 23;81:2.28.1–2.28.12. doi: 10.1002/cpns.35

Figure 3. TrueBlack quenches autofluorescence in double— labeled tissue.

Figure 3

E12.5 fixed tissue sections were treated for citrate antigen retrieval, stained for Sox21 (green) and Pax6 (red) immunofluorescence, and treated with 1X TrueBlack for 3 minutes. Images of the forebrain (Pal= pallidum, PT= prethalamic eminence, TV= telencephalic vesicle) showed red blood cells (arrows) fluorescing across green (488 nm), red (555 nm), and blue (405 nm) wavelengths, which can imitate immunofluorescent signal co— localization. TrueBlack treatment eliminated this red blood cell autofluorescence.