Figure 4. 1X TrueBlack with short incubation time is sufficient.

E12.5 fixed tissue sections were treated with different concentrations of TrueBlack (1X–3X) for 3 or 30 min, and sections of the gut (gt) were imaged at green (488 nm), red (555 nm), and blue (405 nm) wavelengths. Arrows indicate autofluorescent red blood cells, which are reduced but not abolished in unstained TrueBlack treated tissue. Increased concentration did not result in reduced autofluorescence, and longer incubation time introduced background fluorescence (asterisks).