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. Author manuscript; available in PMC: 2018 Oct 23.
Published in final edited form as: Curr Protoc Neurosci. 2017 Oct 23;81:2.28.1–2.28.12. doi: 10.1002/cpns.35

Figure 5. 1X TrueBlack with short incubation time is sufficient for immunofluorescence.

Figure 5

E12.5 fixed tissue sections were immunostained for Peripherin, then treated with different concentrations of TrueBlack (1X–3X) for 3 or 30 min. Sections of the gut (gt) (A) and trigeminal ganglion (Tg) (B) were imaged. Arrows indicate autofluorescent red blood cells, which were drastically diminshed in immunofluorescent TrueBlack treated tissue. Increased concentration or longer incubation time did not result in reduced autofluorescence.