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. Author manuscript; available in PMC: 2017 Oct 26.
Published in final edited form as: Biochemistry. 2016 Nov 2;55(45):6327–6336. doi: 10.1021/acs.biochem.6b00932

Figure 3.

Figure 3

Confocal analysis of the effect of curcumin on TPA-induced membrane translocation of αWT, εWT, α/εC2, α/εC1, ε/αC2 and ε/αC1. The upper panel shows the confocal images of wild type (αWT and εWT) and mutants protein (α/εC2, α/εC1, ε/αC2 and ε/αC1) after cells were treated with TPA (10 μM) and/or curcumin (25 μM) as indicated for 1 h. HEK293 cells were transiently transfected with a particular plasmid and confocal images were acquired as described in Material and Methods section. Control (ctrl) refers to the sample with vehicle (0.1% DMSO) treated cells. The lower panel bar graph shows the protein ratio of cytoplasm to plasma-membrane, quantified from fluorescence intensity. Results are displayed as Mean ± SEM derived from three independent experiments and from three different cells. One-way ANOVA (Bonferroni post hoc test) was used for statistical significance analysis. *, P values less than 0.05 are considered as significant.