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. Author manuscript; available in PMC: 2017 Nov 24.
Published in final edited form as: Nature. 2017 May 24;546(7656):113–117. doi: 10.1038/nature22397

Extended Data Figure 2. Cryo-EM densities for ternary complex in each Structure.

Extended Data Figure 2

(a) Cryo-EM density for ternary complex and codon in Structure I is shown at 3 σ after applying a B-factor of −36 Å2. (b) Cryo-EM density for cognate tRNA and codon in Structure I is shown as in (a). (c) Cryo-EM density for the anticodon and codon, which are not base paired, in Structure I is shown at 4 σ after applying a B-factor of −36 Å2. (d) Cryo-EM density for ternary complex and codon in Structure II is shown at 3 σ after applying a B-factor of −50 Å2. (e) Cryo-EM density for cognate tRNA and codon in Structure II is shown as in (d). (f) Cryo-EM density for the anticodon and codon, which are base paired, in Structure II is shown at 4.5 σ after applying a B-factor of −50 Å2. (g) Cryo-EM density for ternary complex and codon in Structure III is shown at 4 σ after applying a B-factor of −100 Å2. (h) Cryo-EM density for cognate tRNA and codon in Structure II is shown as in (g). (i) Cryo-EM density for the anticodon and codon, which are base paired, in Structure III is shown at 5 σ after applying a B-factor of −150 Å2. (j) Cryo-EM density for ternary complex and codon in Structure I-nc is shown at 3 σ. (k) Cryo-EM density for near-cognate tRNA and codon in Structure I-nc is shown as in (j). (l) Cryo-EM density for the anticodon and codon, which are not base paired, in Structure I-nc is shown at 3.5 σ for T tRNA and 16s rRNA or 4 σ for mRNA. (m) Cryo-EM density for ternary complex and codon in Structure II-nc is shown at 3 σ after applying a B-factor of −25 Å2. (n) Cryo-EM density for near-cognate A*/T tRNA and codon in Structure II-nc is shown as in (m). (o) Cryo-EM density for the anticodon and codon, which are interacting in Structure II-nc is shown at 4.5 σ after applying a B-factor of −25 Å2. (p) Cryo-EM density for ternary complex and codon in Structure III-nc is shown at 4 σ after applying a B-factor of −50 Å2. (q) Cryo-EM density for near-cognate tRNA and codon in Structure II-nc is shown as in (p). (r) Cryo-EM density for the anticodon and codon, which are base paired, in Structure III-nc is shown at 5.2 σ after applying a B-factor of −60 Å2.