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Example optimized sandwich immunoassay with enzymatic signal amplification. The flow rate of each step is 30 μL/min.
| Description | Duration (min) |
|---|---|
| Running buffer | 2 |
| Sample | 20 |
| Running buffer | 2 |
| Secondary antibody | 10 |
| Running buffer | 2 |
| SA-HRP conjugate | 10 |
| Running buffer | 3 |
| 4-CN solution | 15 |
| Running buffer | 7 |
