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. Author manuscript; available in PMC: 2018 Feb 7.
Published in final edited form as: Nat Chem Biol. 2017 Aug 7;13(10):1063–1065. doi: 10.1038/nchembio.2448

Figure 1. SAM is activated as a non-ribosomal peptide synthetase (NRPS) building block and loaded onto the phosphopantetheinyl arm of ClbH.

Figure 1

(a) Pks-dependent, cyclopropane-containing metabolites isolated from pks+ E. coli mutant strains. (b) ATP-[32P]-PPi exchange assay for the substrate specificity of full-length ClbH, ClbH-A1, ClbH-C-A2-PCP, and ClbH-C-A2-PCP-S1524A. C, condensation; A, adenylation; PCP, peptidyl carrier protein. Results of scintillation counting were averaged and scaled relative to the activation of L-Ser by full-length ClbH (data points are shown). Error bars represent the standard error of the mean (s.e.m.) of three replicates.